SELECTED PUBLICATIONS WITH THE MICROSPECTROPHOTOMETER

       

Klink BU, Goody RS, Scheidig AJ. A newly designed microspectrofluorometer for kinetic studies on protein crystals in combination with x-ray diffraction.
BIOPHYSICAL JOURNAL 91 (3): 981-992 AUG 2006

Yeremenko S, van Stokkum IHM, Moffat K, et al. Influence of the crystalline state on photoinduced dynamics of photoactive yellow protein studied by ultraviolet-visible transient absorption spectroscopy.
BIOPHYSICAL JOURNAL 90 (11): 4224-4235 JUN 2006

Baxter RHG, Krausz E, Norris JR. Photoactivation of the photosynthetic reaction center of Blastochloris viridis in the crystalline state.
JOURNAL OF PHYSICAL CHEMISTRY B 110 (2): 1026-1032 JAN 19 2006

Daruzzaman A, Clifton IJ, Adlington RM, et al. Unexpected oxidation of a depsipeptide substrate analogue in crystalline isopenicillin N synthase.
CHEMBIOCHEM 7 (2): 351-358 FEB 2006


Johansson T, Oswald C, Pedersen A, Tornroth S, Okvist M, Karlsson BG, Rydstrom J, Krengel U: X-ray structure of domain I of the proton-pumping membrane protein transhydrogenase from Escherichia coli.

JOURNAL OF MOLECULAR BIOLOGY 352 (2): 299-312 SEP 16 2005


Tubbs JL, Tainer JA, Getzoff ED Crystallographic structures of Discosoma red fluorescent protein with immature and mature chromophores: Linking peptide bond trans-cis isomerization and acylimine formation in chromophore maturation.
BIOCHEMISTRY 44 (29): 9833-9840 JUL 26 2005

Friemann R, Ivkovic-Jensen MM, Lessner DJ, et al. Structural insight into the dioxygenation of nitroarene compounds: the crystal structure of nitrobenzene dioxygenase.

JOURNAL OF MOLECULAR BIOLOGY 348 (5): 1139-1151 MAY 20 2005

Carlsson GH, Nicholls P, Svistunenko D, et al. Complexes of horseradish peroxidase with formate, acetate, and carbon monoxide.

BIOCHEMISTRY 44 (2): 635-642 JAN 18 2005

Pearson AR, Mozzarelli A, Rossi GL Microspectrophotometry for structural enzymology. CURRENT OPINION IN STRUCTURAL BIOLOGY 14 (6): 656-662 DEC 2004

 

Neutze R, Huldt G, Hajdu J, et al. Potential impact of an X-ray free electron laser on structural biology.

RADIATION PHYSICS AND CHEMISTRY 71 (3-4): 905-916 OCT-NOV 2004

 

Mather OC, Singh A, van Boxel GI, et al. Active-site conformational changes associated with hydride transfer in proton-translocating transhydrogenase.

BIOCHEMISTRY 43 (34): 10952-10964 AUG 31 2004

 

Edman K, Royant A, Larsson G, et al. Deformation of helix C in the low temperature L-intermediate of bacteriorhodopsin.

J BIOL CHEM 279 (3): 2147-2158 JAN 16 2004


Schenkl S, Portuondo E, Zgrablic G, et al.: Compositional heterogeneity  reflects partial dehydration in three-dimensional crystals of  bacteriorhodopsin.

J MOL BIOL 329 (4): 711-719 JUN 13 2003

Kort, R; Ravelli, RB; Schotte, F; et al: Characterization of photocycle intermediates in rystalline photoactive yellow protein.

PHOTOCHEM PHOTOBIOL, 78 (2): 131-137 AUG 2003

Wilmot, CM: Oxygen activation in a copper-containing amine oxidase.

BIOCHEM SOC T, 31: 493-496 Part 3 JUN 2003

Ferrari, D; Merli, A; Peracchi, A; et al: Catalysis and electron transfer in protein crystals: the binary and ternary complexes of methylamine dehydrogenase with electron acceptors. BBA-PROTEINS PROTEOM, 1647 (1-2): 337-342 Sp. Iss. SI APR 11 2003

Pearson , AR ; Wilmot, CM: Catching catalysis in the act: using single crystal kinetics to trap methylamine dehydrogenase reaction intermediates.

BBA-PROTEINS PROTEOM, 1647 (1-2): 381-389 Sp. Iss. SI APR 11 2003

Kaiser, JT; Bruno, S; Clausen, T; et al: Snapshots of the cystine lyase C-DES during
catalysis - Studies in solution and in the crystalline state.

J BIOL CHEM, 278 (1): 357-365 JAN 3 2003

Weik, M; Berges, J; Raves, ML; et al: Evidence for the formation of disulfide radicals in protein crystals upon X-ray irradiation.

J SYNCHROTRON RADIAT, 9: 342-346 Part 6 NOV 2002

Murray, J; Garman, E: Investigation of possible free-radical scavengers and metrics for radiation damage in protein cryocrystallography.

J SYNCHROTRON RADIAT, 9: 347-354 Part 6 NOV 2002

Phillips, RS; Demidkina, TV; Zakomirdina, LN; et al: Crystals of tryptophan indole-lyase and tyrosine phenol-lyase form stable quinonoid complexes.

J BIOL CHEM, 277 (24): 21592-21597 JUN 14 2002

Bourgeois, D; Vernede, X; Adam, V; et al: A microspectrophotometer for UV-visible absorption and fluorescence studies of protein crystals.

J APPL CRYSTALLOGR, 35: 319-326 Part 3 JUN 2002

Okada, T; Fujiyoshi, Y; Silow, M; et al: Functional role of internal water molecules in rhodopsin revealed by x-ray crystallography.

P NATL ACAD SCI USA , 99 (9): 5982-5987 APR 30 2002

Sakai , K; Matsui, Y; Kouyama, T; et al: Optical monitoring of freeze-trapped reaction intermediates in protein crystals: a microspectrophotometer for cryogenic protein crystallography.

J APPL CRYSTALLOGR, 35: 270-273 Part 2 APR 2002

Ursby, T; Weik, M; Fioravanti, E; et al: Cryophotolysis of caged compounds: a technique for trapping intermediate states in protein crystals.

ACTA CRYSTALLOGR D, 58: 607-614 Part 4 APR 2002

Berglund, G.I., Carlsson, G.H., Smith, A.T., Szoke, H., Henriksen, A. & Hajdu, J. (2002) The catalytic pathway of horseradish peroxidase at high resolution.

NATURE 417, 463-468.

Wilmot, C.M., Sjogren, T., Carlsson, G.H., Berglund, G.I. & Hajdu, J. (2002). Defining redox state of X-ray crystal structures by single-crystal ultraviolet-visible microspectrophotometry.

METHODS IN ENZYMOLOGY, "Redox Cell Biology and Genetics, part B", 353, pp 301-318, Ed. C. K. Sen & L. Packer, Academic Press ( USA ).

Edman K, Royant A, Nollert P, et al.: Early structural rearrangements in the photocycle of an integral membrane sensory receptor.

STRUCTURE 10(4): 473-482 APR 2002

Neutze R, Pebay-Peyroula E, Edman K, et al.: Bacteriorhodopsin: a high-resolution structural view of vectorial proton transport.

BBA-BIOMEMBRANES 1565(2): 144-167 OCT 11 2002

Bourgeois D, Vernede X, Adam V, et al.: A microspectrophotometer for UV-visible absorption and fluorescence studies of protein crystals.

J APPL CRYSTALLOGR 35: 319-326 Part 3 JUN 2002


Wilmot CM, Pearson AR : Cryocrystallography of metalloprotein reaction intermediates.

CURR OPIN CHEM BIOL 6 (2): 202-207 APR 2002

Sjögren, T., Carlsson, G., Larsson, G., Hajdu, A., Andersson, C., Pettersson, H., Hajdu, J. (2002) Protein crystallography in a vapour stream: data collection, reaction initiation, and intermediate trapping in naked, hydrated protein crystals.

J. APPL. CRYST. 35, 113-116.

Specht, A; Ursby, T; Weik, M; et al: Cryophotolysis of ortho-nitrobenzyl derivatives of enzyme ligands for the potential kinetic crystallography of macromolecules. CHEMBIOCHEM, 2 (11): 845-+ NOV 5 2001

Kavanaugh, JS; Weydert, JA; Rogers, PH; et al: Site-directed mutations of human hemoglobin at residue 35 beta: A residue at the intersection of the alpha 1 beta 1, alpha 1 beta 2, and at alpha 1 alpha 2 interfaces.

PROTEIN SCI, 10 (9): 1847-1855 SEP 2001

 

Marabotti, A; De Biase, D; Tramonti, A; et al: Allosteric communication of tryptophan synthase - Functional and regulatory properties of the beta S178P mutant.

J BIOL CHEM, 276 (21): 17747-17753 MAY 25 2001

Patel, SS; Pittman, RN: Hemoglobin microspectrophotometry in three dimensions.

FASEB J, 15 (4): A39-A39 Part 1 MAR 7 2001

Bruno, S; Schiaretti, F; Burkhard, P; et al: Functional properties of the active core of human cystathionine beta-synthase crystals.

J BIOL CHEM, 276 (1): 16-19 JAN 5 2001

Adolph, HW; Zwart, P; Meijers, R; et al: Structural basis for substrate specificity differences
of horse liver alcohol dehydrogenase isozymes.

BIOCHEMISTRY-US, 39 (42): 12885-12897 OCT 24 2000

Bruno, S; Bettati, S; Manfredini, M; et al: Oxygen binding by alpha(Fe2+)(2)beta(Ni2+)(2) hemoglobin crystals.

PROTEIN SCI, 9 (4): 683-692 APR 2000

Royant A, Edman K, Ursby T, et al.: Spectroscopic characterization of bacteriorhodopsin's L-intermediate in 3D crystals cooled to 170 K.

PHOTOCHEM PHOTOBIOL 74 (6): 794-804 DEC 2001

Stoddard BL: Trapping reaction intermediates in macromolecular crystals for structural analyses: 

METHODS 24 (2): 125-138 JUN 2001

Sjögren, T., Hajdu, J. (2001) Structure of the bound dioxygen species in the cytochrome oxidase reaction of cytochrome cd1 nitrite reductase.

J. BIOL.CHEM. 276, 13072­13076.

Sjögren, T., Hajdu, J. (2001) The structure of an alternative form of Paracoccus pantotrophus cytochrome cd(1) nitrite reductase.

J. BIOL.CHEM. 276, 29450-29455.

Schlichting I: Crystallographic structure determination of unstable species.

ACCOUNTS CHEM RES 33 (8): 532-538 AUG 2000

Royant A, Edman K, Ursby T, et al.: Helix deformation is coupled to vectorial proton transport in the photocycle of bacteriorhodopsin.

NATURE 406 (6796): 645-648 AUG 10 2000 .
                                     

Sjögren, T., Svensson-Ek, M., Hajdu, J., Brzezinski, P.: Proton-Coupled  Structural Changes upon Binding of CO to Cytochrome cd1: a combined flash photolysis and X-ray crystallography study.

BIOCHEMISTRY 39, 10967-10974 (2000).

Ren, Z., Bourgeois, D., Helliwell, J.R., Moffat, K., Srajer, V., Stoddard, B.L. (1999) Laue crystallography: coming of age.

J. SYNCHR. RAD., 6, 891-917.

Burzlaff, N.I., Rutledge, P.J., Clifton , I.J., Hensgens, C.M.H., Pickford, M., Adlington, R.M.,
Roach, P.L., Baldwin , J.E. (1999) The reaction cycle of isopenicillin N synthase observed by X-ray diffraction.

NATURE, 401, 721-724.

Wilmot, C. M., Hajdu, J., Saysell, C. G., McPherson, M. J., Knowles, P. F.,
Phillips, S. E. V. (1999) A movie of catalysis in the copper-containing quinoenzyme amine oxidase.

J. INORG.BIOCHEM., 1999, Vol. 74, No.1-4, p.339


Edman, K., Nollert, P., Royant, A., Belrhali, H., Pebay-Peyroula, E., Hajdu, J, Neutze, R. & Landau, E. M. (1999) High-resolution X-ray structure of an early intermediate in the bacteriorhodopsin photocycle.

NATURE, 401, 822-826.

Wilmot, C. M., Hajdu, J., McPherson, M. J., Knowles, P. F., Phillips, S. E. V. (1999)
Visualization of Dioxygen Bound to Copper During Enzyme Catalysis.

SCIENCE, 286, 1724-1728.

Genick , U.K. , Soltis, S.M., Kuhn, P., Canestrelli, I.L., Getzoff, E.D. (1998) Structure at 0.85 angstrom resolution of an early protein photocycle intermediate.

NATURE, 392, 206-209.

Moffat, K. (1998) Time-resolved crystallography. ACTA CRYST. A54, 833- 841.
Roach, P.L., Clifton , I.J., Hensgens, C.M.H., Shibata, N., Schofield, C.J., Hajdu, J.,
Baldwin , J.E. (1997) Structure of isopenicillin N synthase complexed with substrate and the mechanism of penicillin formation.

NATURE, 387, 827-830.

Schlichting, I. , Goody, R.S. (1997) Triggering methods in crystallographic enzyme kinetics.

METHODS IN ENZYMOLOGY, 277, 467-490.

Williams, P. A., Fülöp, V., Garman, E., Saunders, N., Ferguson , S. J. and Hajdu, J. (1997)
Haem ligand-switching during catalysis in crystals of a nitrogen cycle enzyme. NATURE, 389, 406-412.

Jouve, H.M., Andreoletti, P., Gouet, P., Hajdu, J., Gagnon, J. (1997) Structural analysis of compound I in hemoproteins: Study on Proteus mirabilis catalase.

BIOCHIMIE, 79, 667-671.
                                    

Brubaker, M.J., Dyer, D.H., Stoddard, B., Koshland, D.E. (1996) Synthesis, kinetics, and structural studies of a photolabile caged isocitrate: A catalytic trigger for isocitrate dehydrogenase.

BIOCHEMISTRY, 35, 2854-2864.

Mozzarelli, A., Rossi, G.L. (1996) Protein function in the crystal.

ANNU. REV. BIOPHYS. BIOMOL. STRUCT., 25, 343-365.

Stoddard, B.L. (1996) Intermediate trapping and Laue X-ray diffraction: Potential for
enzyme mechanism, dynamics, and inhibitor screening.

PHARM. THERAP., 70, 215-256.

Helliwell, J.R., Helliwell, M. (1996) X-ray crystallography in structural chemistry and
molecular biology.

CHEM. COMMUN., No.14, 1595-1602

Chayen, N.E., Boggon, T.J., Cassetta, A., Deacon, A., Gleichmann, T., Habash, J.,
Harrop, S.J., Helliwell, J.R., Nieh, Y.P., Peterson, M.R., Raftery, J., Snell, E.H., Hadener, A., Niemann, A.C., Siddons, D.P., Stojanoff, V., Thompson, A.W., Ursby, T., Wulff, M. (1996) Trends and challenges in experimental macromolecular crystallography.

QUART. REV.BIOPHYS., 29, 227-278.

Mancia, F., Keep, N.H., Nakagawa, A., Leadlay, P.F., Mc Sweeney, S., Rasmussen, B.,
Bosecke, P., Diat, O., Evans, P.R. (1996) How coenzyme B-12 radicals are generated the crystal-structure of methylmalonyl-coenzyme-A mutase at 2 Ångstrom resolution.

STRUCTURE, 4, 339-350.

Gouet, P., Jouve, H.-M., Williams, P. A., Andersson, I. , Andreoletti, P., Nussaume, L.
and Hajdu, J. (1996) Ferryl intermediates of catalase captured by time-resolved Weissenberg crystallography and UV-VIS spectroscopy.

NATURE STRUCTURAL BIOLOGY, 3, 951-956.

Srajer, V., Teng, T., Ursby, T., Pradervand, C., Ren, Z., Adachi, S., Schildkamp, W.,
Bourgeois, D., Wulff, M. and Moffat, K. (1996) Photolysis of the carbon-monoxide complex of myoglobin - nanosecond time-resolved crystallography.

SCIENCE 274, 1726-1729.

Peach, J.M., Pratt, A.J., Snaith, J.S. (1995) Photolabile benzoin and furoin esters of a
biologically-active peptide.

TETRAHEDRON, 51, 10013-10024.

Stoddard, B.L., Farber, G.K. (1995) Direct measurement of reactivity in the protein crystal
by steady- state kinetic-studies.

STRUCTURE, 3, 991-996.

O¹Hara, P., Goodwin, P., Stoddard, B.L. (1995) Direct measurement of diffusion rates in
enzyme crystals by video absorbance spectroscopy.

J. APPL. CRYST., 28, 829-834.

Hadfield, A. T. and Hajdu, J. (1994) On the photochemical release of phosphate from 3,5-dinitrophenyl phosphate in a protein crystal.

J. MOL. BIOL., 236, 995-1000.

Fülöp, V., Phizackerley, P. Soltis, M., Clifton , I. J., Wakatsuki, S., Erman, J., Hajdu, J.
and Edwards, S. L. (1994) Laue diffraction study on the structure of cytochrome c peroxidase compound I.

STRUCTURE, 2, 201-208.

Duke, E.M.H., Wakatsuki, S., Hadfield, A., Johnson, L.N. (1994) Laue and monochromatic diffraction studies on catalysis in phosphorylase-b crystals.

PROTEIN SCIENCE, 3, 1178-1196.

Hadfield, A. T. and Hajdu, J. (1993) A fast and portable micro-spectrophotometer for
time-resolved X-ray diffraction experiments.

J. APPL. CRYST., 26, 839-842.




 

 

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